https://pustaka-psm.unilak.ac.id/index.php/jip <p>Jurnal Ilmiah Pertanian (JIP) is an open-access journal that publishes original research articles on agricultural advances and various other agricultural resources at the local level to increase global awareness.. <br><br>Initially, the Jurnal Ilmiah Pertanian (JIP) was published twice yearly, every February and August. However, starting with Volume 19, 2022, Jurnal Ilmiah Pertanian (JIP) is a triannual journal published every March, July, and Dec.&nbsp; It is published electronically via the journal website. ISSN (Print) 1829-8346 ISSN (Online) 2502-5988</p> Universitas Lancang Kuning en-US Jurnal Ilmiah Pertanian 1829-8346 https://pustaka-psm.unilak.ac.id/index.php/jip/article/view/17579 <p>Winged-bean meal can serve as a substitute for soybean meal in plant-based fish feed, despite its trypsin-inhibitor properties, which can be alleviated through heating processes. This study aimed to explore the potential of winged-bean meal subjected to different heating methods, namely oven (110 °C for 30 minutes) and autoclave (121 °C for 30 minutes), based on proximate analysis and trypsin activity. The study applied three treatments with four replications: oven heating (O), autoclave heating (A), and control (without heating, E). All data were analyzed using analysis of variance and Duncan’s multiple range test to determine the best treatment. Data were also compared to the nutrient requirements of several cultured freshwater fish in Indonesia. Heating treatments improved <em>in vitro</em> trypsin activity (1300 units/O and 1135 units/A vs. 835 units) but reduced protein (29.05±1.96%/A and 31.73±1.52%/O vs. 35.17±0.38%) and moisture contents. Additionally, the O treatment met the standards for aquafeed protein content (27-45%) and potentially fulfilled the protein requirements for catfish (25-40%), Nile tilapia (30-35%), and Java barb (30-32%). Therefore, oven heating presents a viable candidate as an alternative plant-based aquafeed ingredient. Nevertheless, the in vivo application of winged-bean meal as an aquafeed ingredient should be further evaluated to determine whether heating treatment effectively enhances digestive activity, nutrient retention, and growth performance in target fish species.</p> Shobrina Silmi Qori Tartila Tholibah Mujtahidah Muh. Azril Anjali Apta Pramudita Lusi Septiani Bagas Putra Satria Ignatius Abel Cristanto Muhammad Abizar Sakti Oktavian Sultan Jibran Risqulloh Santika Dewi Astuti Copyright (c) 2024 Jurnal Ilmiah Pertanian https://creativecommons.org/licenses/by/4.0 2024-07-02 2024-07-02 21 2 10.31849/jip.v21i2.17579